Speaker: Lu Bo
Affiliation: School of Physics, Peking University, China
Abstract Details: Voltage-biased solid-state nanopores are well established in their ability to detect and characterize single polymers, such as DNA, in electrolytes. The addition of a pressure gradient across the nanopore yields a second, independent molecular driving force that provides new freedom for studying molecules in nanopores. We show that opposing pressure- and voltage-derived forces allow us to detect and resolve very short DNA molecules, as well as to detect near-neutral polymer strands. Moreover, by simply balancing pressure- and voltage-derived forces in the nanopore, we were able to detect single biomolecule charge density. The measurement of the charge density of ds-DNA from pH 4 to 10 shows that the charge density of dsDNA drops significantly below pH 6, while the effective hydrodynamic radius of the DNA decreases due to the shedding of the immobilized Stern-like layer around the DNA molecule. The technique presented here is more informative and convenient for surface charge detection than the current commercial systems based on isoelectric focusing (IEF).Click HERE for directions

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