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Biological function of graphene and its derivatives on inducing osteogenic and odontogenic differentiation in dental mesenchymal stem cells

Speaker: 
Xie Han
Date: 
Fri, 26/08/2016 - 12:15pm to 1:55pm
Location: 
S16 Level 6 - Theory conference room
Event Type: 
Seminars

Abstract: Graphene has shown the ability in inducing osteogenic differentiation of stem cells. However, the cellular mechanisms involved in this process remain unexplored. Our studies are investigating the biological function of graphene and its derivatives (2D graphene-2DGp, 3D graphene-3DGp and graphene oxide-GO) during their osteogenic and odontogenic differentiation in dental stem cells. All the graphene substrates supported cell attachment and proliferation, and induced osteogenic differentiation of both periodontal stem cells (PDLSC) and dental pulp stem cells (DPSC) into mature osteoblasts with a higher level as compared with glass slide (GL) and polystyrene scaffold (PS). Bone-related genes and proteins (COL I, RUNX2 and OCN) were upregulated regardless the use of osteogenic medium. Remarkably, GO enhanced odontogenic differentiation as well by upregualting dentin-related genes (MSX-1, PAX-9, DMP-1), which was not observed from 2DGp. Meanwhile, stiffness sensitive genes MHY10 and MHY10-V2 were evaluated, and those higher expressions on 2D and 3D graphene suggest that both chemical and physical properties of graphene act synergistically while ruling osteoblastic differentiation of stem cells. These findings broaden the use of graphene substrates in combination with dental materials to improve their bioactivity and beyond.

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